Chapter title |
Immortalization of Murine Bone Marrow-Derived Macrophages
|
---|---|
Chapter number | 4 |
Book title |
Macrophages
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7837-3_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7836-6, 978-1-4939-7837-3
|
Authors |
Dominic De Nardo, Dhan V. Kalvakolanu, Eicke Latz |
Abstract |
Macrophages are specialized phagocytes that display a variety of important functions for the host immune system. They are particularly important for the recognition of exogenous and endogenous danger signals, forming the defensive front line as part of innate immune response. As such, murine macrophages are commonly used for in vitro cell-based assays examining the mechanisms of innate immune activation, which can require the ongoing breeding and housing of a large number of genetically modified mouse strains. Here, we describe a robust protocol for the generation of immortalized bone marrow-derived macrophages (iBMDMs) from primary murine bone marrow cells. We further provide general protocols for harvesting, freezing, and thawing of bone marrow cells, maintaining iBMDMs in culture and generation of monoclonal iBMDM populations by single-cell cloning. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 24% |
Student > Bachelor | 8 | 15% |
Researcher | 7 | 13% |
Student > Doctoral Student | 5 | 9% |
Student > Master | 5 | 9% |
Other | 6 | 11% |
Unknown | 10 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 24% |
Immunology and Microbiology | 13 | 24% |
Agricultural and Biological Sciences | 8 | 15% |
Medicine and Dentistry | 5 | 9% |
Environmental Science | 1 | 2% |
Other | 2 | 4% |
Unknown | 12 | 22% |