Cell Culture Engineering VI

Chapter 1 Cell therapy in kidney failure

Chapter 2 Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE)

Chapter 3 Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination

Chapter 4 Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line

Chapter 5 Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them

Chapter 6 An overview of viral and viral-like agents in cell culture systems

Chapter 7 New adenovirus vectors for protein production and gene transfer

Chapter 8 Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue

Chapter 9 Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth — associated production of a foreign protein, β-galactosidase

Chapter 10 A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy

Chapter 11 Recombinant insulin-like growth factor-I (IGF-I) production in Super -CHO results in the expression of IGF-I receptor and IGF binding protein 3

Chapter 12 Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions

Chapter 13 Regulated multicistronic expression technology for mammalian metabolic engineering

Chapter 14 Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions

Chapter 15 Historical reflections on cell culture engineering

Chapter 16 Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation

Chapter 17 Population balance model of in vivo neutrophil formation following bone marrow rescue therapy

Chapter 18 Mammalian cell retention devices for stirred perfusion bioreactors

Chapter 19 Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells

Chapter 20 Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions

Chapter 21 Effect of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures

Chapter 22 Effects of CO 2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production

Chapter 23 pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons