Rapid Cycle Real-Time PCR — Methods and Applications

Chapter 1 Housekeeping Genes: A Gold Standard?

Chapter 2 The Choice of House Keeping Genes in MRD-Quantification of AML1-ETO Positive Acute Myeloid Leukemia

Chapter 3 Quantification of mRNA Using Linear Regression of Log-Linear PCR Data-Points as an Alternative for the Standard Curve Approach

Chapter 4 Measuring Genome Sizes by Absolute Quantification

Chapter 5 Relative Quantification of Insulin Gene Expression on the LightCycler Using SYBR Green I

Chapter 6 Quantification of δRec-ψJα Signal Joint T-Cell Receptor Excision Circle DNA in Patients after Autologous and Allogeneic Stem Cell Transplantation

Chapter 7 Expression Analysis of Mitochondrial Components in a Variety of Plant Species Using Real-Time Quantitative PCR

Chapter 8 Quantification of Ikaros Splice Variants by Real-Time PCR

Chapter 9 Methods to Quantify Cytokine Gene Expression by Real-Time PCR

Chapter 10 Profiling Breast Cancer Using Real-Time Quantitative PCR

Chapter 11 HER-2/neu Gene Copy Number Quantified by Real-Time PCR in Cell Lines and Breast Cancer Tissue

Chapter 12 Quantification of Several Components of Fibrinolytic and Matrix Metalloproteinase Systems in Primary Breast Cancer

Chapter 13 A Rapid Cycle Real-Time Quantitative β-globin PCR for Quantification of Human DNA in Feces

Chapter 14 Quantification of Tumor Load for Follicular Lymphoma by Quantifying BCL2/IGH Using Real-Time Quantitative PCR by LightCycler™

Chapter 15 Real-Time Quantification of the AML Rearrangements, AML1-ETO and TEL-AML1 , in Acute Leukemia

Chapter 16 Rapid Detection of Gene Duplications in Charcot-Marie-Tooth 1A Disease by SNP Genotyping Using Real-Time PCR

Chapter 17 Cytochrome P450 2D6 Deletion Genotyping Using Derivative Curve Analysis on the LightCycler

Chapter 18 Trisomy 21 Detected by SNP Allele Ratios

Chapter 19 Quantitative Chimerism Analysis by Allele-Specific Real-Time PCR of a 10 bp Insertion/Deletion Polymorphism within the Promotor Region of Factor Vllc

Chapter 20 Detection of SARS-Coronavirus in the LightCycler by 5′-Nuclease Real-Time RT-PCR

Chapter 21 Normalized Quantitative Rapid-Cycle Real-Time PCR for the Assessment of CMV-DNA Copies