Chapter title |
Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throughput DNA Sequencing.
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Chapter number | 17 |
Book title |
Immunosenescence
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Published in |
Methods in molecular biology, January 2015
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DOI | 10.1007/978-1-4939-2963-4_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2962-7, 978-1-4939-2963-4
|
Authors |
Wang, Chen, Liu, Yi, Roskin, Krishna M, Jackson, Katherine J L, Boyd, Scott D, Chen Wang, Yi Liu, Krishna M. Roskin, Katherine J. L. Jackson, Scott D. Boyd, Roskin, Krishna M., Jackson, Katherine J. L., Boyd, Scott D. |
Abstract |
High-throughput DNA sequencing techniques have greatly accelerated the pace of research into the repertoires of antibody and T cell receptor gene rearrangements that confer antigen specificity to adaptive immune responses. Studies of aging-related changes in human B cell repertoires have benefited from the ability to detect and quantify thousands to millions of B cell clones in human samples, and study the mutational lineages and isotype switching relationships within each clonal lineage. Correlation of repertoire analysis with antibody gene data from antigen-specific B cells is poised to give much greater insight into clinically relevant B cell responses and memory storage. Here, we describe strategies for preparing and analyzing human antibody gene libraries for studying B cell repertoires. |
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Unknown | 3 | 20% |