Chapter 1 Glycan Microarray Screening Assay for Glycosyltransferase Specificities

Chapter 2 A Fluorescence-Based Assay for Core 1 β3Galactosyltransferase (T-Synthase) Activity

Chapter 3 Structural and Biochemical Analysis of a Bacterial Glycosyltransferase

Chapter 4 Study of the Biological Functions of Mucin Type Core 3 O-glycans.

Chapter 5 Generation of Anti-sulfated Glycan Antibodies Using Sulfotransferase-Deficient Mice

Chapter 6 Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells

Chapter 7 A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence–Function Relationships of Golgi-Glycosyltransferases

Chapter 8 Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans During Development

Chapter 9 Photoaffinity Labeling of Protein O -Mannosyltransferases of the PMT1/PMT2 Subfamily

Chapter 10 Enzymatic Analysis of the Protein O -Glycosyltransferase, Rumi, Acting Toward Epidermal Growth Factor-Like (EGF) Repeats

Chapter 11 Enzymatic Characterization of Recombinant Enzymes of O -GlcNAc Cycling

Chapter 12 Antibodies and Activity Measurements for the Detection of O -GlcNAc Transferase and Assay of its Substrate, UDP-GlcNAc

Chapter 13 In Vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases

Chapter 14 In Vitro UDP-Sugar:Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography

Chapter 15 In vitro O-antigen ligase assay.

Chapter 16 Functional Identification of Bacterial Glucosyltransferase WbdN

Chapter 17 Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains

Chapter 18 Methods for Measuring Class I Membrane-Bound Hyaluronan Synthase Activity

Chapter 19 Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei

Chapter 20 Mannose-6-Phosphate: A Regulator of LLO Destruction

Chapter 21 N -Acetylglucosaminyltransferase (GnT) Assays Using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb)

Chapter 22 A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4 N -Acetylglucosaminyltransferase VI Activity

Chapter 23 In Vitro Assays of Orphan Glycosyltransferases and Their Application to Identify Notch Xylosyltransferases

Chapter 24 In Vitro Folding of β-1,4Galactosyltransferase and Polypeptide-α- N -Acetylgalactosaminyltransferase from the Inclusion Bodies

Chapter 25 An Assay for α 1,6-Fucosyltransferase (FUT8) Activity Based on the HPLC Separation of a Reaction Product with Fluorescence Detection

Chapter 26 Immunodetection of Glycosyltransferases in Gastrointestinal Tissues

Chapter 27 Glycosyltransferases in Chemo-enzymatic Synthesis of Oligosaccharides

Chapter 28 Tumor targeting by a carbohydrate ligand-mimicking Peptide.

Chapter 29 Glycoengineering of Human Cell Lines Using Zinc Finger Nuclease Gene Targeting: SimpleCells with Homogeneous GalNAc O-glycosylation Allow Isolation of the O-glycoproteome by One-Step Lectin Affinity Chromatography

Chapter 30 Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases

Chapter 31 Erratum: Study of the Biological Functions of Mucin Type Core 3 O -glycans