Chapter title |
Isolation and short-term culture of mouse spermatocytes for analysis of meiosis.
|
---|---|
Chapter number | 17 |
Book title |
Meiosis
|
Published in |
Methods in molecular biology, January 2009
|
DOI | 10.1007/978-1-60761-103-5_17 |
Pubmed ID | |
Book ISBNs |
978-1-60761-102-8, 978-1-60761-103-5
|
Authors |
La Salle S, Sun F, Handel MA, Sophie La Salle, Fengyun Sun, Mary Ann Handel, Salle, Sophie La, Sun, Fengyun, Handel, Mary Ann |
Abstract |
Understanding meiosis is facilitated by in vitro experimental approaches, but this has not been easily applicable to mammalian meiocytes. Available methods for in vitro analysis of mammalian oocytes are generally limited to experimental analysis of the late prophase period. Short-term cultures of male germ cells have been useful for analysis of earlier meiotic prophase pathways, as well as onset of the meiotic division phase, but no studies have achieved reliable spermatogenesis in vitro. Here we describe a method for preparing highly enriched pachytene spermatocytes from mouse testicular cell suspensions using cell-size fractionation by sedimentation through a bovine serum albumin gradient at unit gravity. We also provide a procedure for short-term culture of spermatocytes and the pharmacological induction of the prophase-to-division phase transition. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Finland | 1 | 2% |
United States | 1 | 2% |
Unknown | 47 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 17 | 35% |
Student > Ph. D. Student | 8 | 16% |
Student > Bachelor | 3 | 6% |
Professor > Associate Professor | 3 | 6% |
Student > Master | 3 | 6% |
Other | 6 | 12% |
Unknown | 9 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 18 | 37% |
Agricultural and Biological Sciences | 15 | 31% |
Medicine and Dentistry | 2 | 4% |
Earth and Planetary Sciences | 1 | 2% |
Unknown | 13 | 27% |