Chapter 2 SUMO fusion technology for enhanced protein expression and purification in prokaryotes and eukaryotes.

Chapter 3 Molecular and Chemical Chaperones for Improving the Yields of Soluble Recombinant Proteins

Chapter 4 Genetic Selection of Solubility-Enhanced Proteins Using the Twin-Arginine Translocation System

Chapter 5 Protein Folding Liquid Chromatography

Chapter 6 Site-Specific Protein Labeling by Intein-Mediated Protein Ligation

Chapter 7 Efficient Expression of Human Aromatase (CYP19) in E. coli

Chapter 8 Expression of Recombinant Cytochromes c in E. coli

Chapter 9 Semi-synthesis of Glycoproteins from E. coli Through Native Chemical Ligation

Chapter 10 Expression of Recombinant Proteins with Uniform N-Termini

Chapter 11 Recent Developments in Difficult Protein Expression: A Guide to E. coli Strains, Promoters, and Relevant Host Mutations

Chapter 12 Periplasmic chaperones used to enhance functional secretion of proteins in E. coli.

Chapter 13 Engineering Unusual Amino Acids into Peptides Using Lantibiotic Synthetase

Chapter 14 Heterologous Gene Expression in E.coli

Chapter 15 Detection of Protein–Protein Interactions in Bacteria by GFP-Fragment Reconstitution

Chapter 16 Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner.

Chapter 17 Introducing Predetermined Mutations Throughout a Target Gene Using TDEM (Transposon-Directed Base-Exchange Mutagenesis)

Chapter 18 Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase