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RT-PCR Protocols

Overview of attention for book
Cover of 'RT-PCR Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology
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    Chapter 2 Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer
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    Chapter 3 Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR
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    Chapter 4 Quantitative RT-PCR Methods for Mature microRNA Expression Analysis
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    Chapter 5 Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction
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    Chapter 6 Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR
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    Chapter 7 Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR
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    Chapter 8 RT-PCR Protocols
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    Chapter 9 Detection of antisense RNA transcripts by strand-specific RT-PCR.
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    Chapter 10 RT-PCR Amplification and Cloning of Large Viral Sequences
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    Chapter 11 One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification
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    Chapter 12 Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR
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    Chapter 13 Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction
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    Chapter 14 The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes.
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    Chapter 15 The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification
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    Chapter 16 Skeletal Muscle RNA Extraction in Preparation for RT-PCR
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    Chapter 17 Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay
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    Chapter 18 Primer Design for RT-PCR
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    Chapter 19 Hot Start PCR
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    Chapter 20 Real-time RT-PCR for Automated Detection of HIV-1 RNA During Blood Donor Screening
Attention for Chapter 18: Primer Design for RT-PCR
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Chapter title
Primer Design for RT-PCR
Chapter number 18
Book title
RT-PCR Protocols
Published in
Methods in molecular biology, January 2010
DOI 10.1007/978-1-60761-629-0_18
Pubmed ID
Book ISBNs
978-1-60761-628-3, 978-1-60761-629-0
Authors

Kelvin Li, Anushka Brownley, Li, Kelvin, Brownley, Anushka

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 156 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 <1%
Netherlands 1 <1%
Kenya 1 <1%
India 1 <1%
Mexico 1 <1%
Nigeria 1 <1%
Unknown 150 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 34 22%
Student > Bachelor 22 14%
Student > Master 13 8%
Researcher 10 6%
Student > Doctoral Student 7 4%
Other 21 13%
Unknown 49 31%
Readers by discipline Count As %
Agricultural and Biological Sciences 56 36%
Biochemistry, Genetics and Molecular Biology 26 17%
Immunology and Microbiology 6 4%
Medicine and Dentistry 5 3%
Unspecified 4 3%
Other 13 8%
Unknown 46 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 March 2021.
All research outputs
#17,662,167
of 25,889,720 outputs
Outputs from Methods in molecular biology
#6,150
of 14,397 outputs
Outputs of similar age
#146,549
of 175,496 outputs
Outputs of similar age from Methods in molecular biology
#103
of 135 outputs
Altmetric has tracked 25,889,720 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,397 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 175,496 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 135 others from the same source and published within six weeks on either side of this one. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.