Methods in Protein Sequence Analysis · 1986

Table of Contents

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   Book Overview
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   Chapter 1 New Ways to Look at Old Proteins
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   Chapter 2 The Development of a Facility to Analyze and Synthesize Genes and Proteins
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   Chapter 3 The Interaction between Molecular Biology and Protein Chemistry
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   Chapter 4 The Synergism of Protein Chemistry and Recombinant DNA Techniques
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   Chapter 5 The Evolution of the Regulatory Response to Products of Recombinant DNA Technology
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   Chapter 6 Analysis of Recombinant Proteins — Current Trends and Practical Limits in Analytical Stringency
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   Chapter 7 Methods for Protein Mutagenesis Using Molecular Biology
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   Chapter 8 The Growing Role of Mass Spectrometry
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    Chapter 9 Application of 252 Cf-Plasma Desorption Mass Spectrometry in Protein Primary Structure Determination
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    Chapter 10 Protein Sequencing by Tandem Mass Spectrometry
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    Chapter 11 Strategies for the Analysis of the Structure and Function of Cell Surface Receptors
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    Chapter 12 Human von Willebrand Factor and the Problems in Sequence Analysis of a 280 K Dalton Protein
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    Chapter 13 Covalent Chromatography, A Powerful Tool in Sequence Studies of Membrane Proteins
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    Chapter 14 The Role of Microbore HPLC in the Purification of Subnanomole Amounts of Polypeptides and Proteins for Gas-Phase Sequence Analysis
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    Chapter 15 Amino Acid Analysis of Submicrogram Hydrolyzate Samples
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    Chapter 16 Automated Solid Phase-Sequencing Using Fluorescent Edman Reagents
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    Chapter 17 Protein-Blotting from SDS-Polyacrylamide Gels on Glass-Fiber Sheets Coated with Quaternized Ammonium Polybases
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    Chapter 18 Sequence Analysis of Proteins from Whole-Cell Lysates After Separation in Analytical Two-Dimensional Gels
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    Chapter 19 Sequencing Proteins from Two-Dimensional Gels Using a Liquid Pressure Extraction Technique
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    Chapter 20 An Improved Method for Electroblotting of Protein from SDS-PAGE Gels for Direct Microsequencing
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    Chapter 21 Direct Sequence Analysis of Proteins after Electroblotting from SDS/Polyacrylamide Gel Separations
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    Chapter 22 Research Resource Facility Satellite Meeting
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    Chapter 23 Microheterogeneity Problems in Protein Sequence Analysis
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    Chapter 24 Generation of Starting Points for Microsequencing with a Protease Specific for the Amino Side of Aspartyl Residues
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    Chapter 25 Proteolytic Cleavage in RP-HPLC Solvents
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    Chapter 26 Rapid Generation of Peptides for N-Terminal Sequence Analysis Using Immobilized Pronase
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    Chapter 27 C-Terminal Sequence of Proteins: Rapid Isolation and Edman Sequencing of C-Terminal Peptides from Digests
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    Chapter 28 Carboxy Terminal Sequence Determination of Peptides and Proteins
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    Chapter 29 Automated Amino Acid Sequence Assignment: Development of a Fully Automated Protein Sequencer Using Edman Degradation
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    Chapter 30 Solid-Phase Microsequencing in Packed Porous Glass Columns: Strategies and Potential for Practical Sequence Analysis
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    Chapter 31 Application of Covalently Modified Glass Fibre Supports to Microsequence Analysis
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    Chapter 32 Multi Purpose Berlin Sequencer Unit Construction of a Multiple Purpose Machine Assembled as Liquid-, Solid-, or Gas-Phase Sequencer
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    Chapter 33 Programmed Cleavage at Proline: Application to the Sequence Determination of Wheat Storage Proteins
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    Chapter 34 Structural Analysis of Glycoproteins: Identification of Carbohydrates and Other Posttranslational Modifications by Mass Spectrometry
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    Chapter 35 Protein Sequence Analysis of Highly-Glycosylated Proteins: Carcinoembryonic Antigen (CEA) as a Model System
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    Chapter 36 Identification of Iodinated Amino Acids in Peptide Sequences of Thyroglobulin
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    Chapter 37 Methods of Separating, Detecting, Hydrolyzing and Storing Fluorotyrosine, Mono-Iodotyrosine and Di-Iodotyrosine
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    Chapter 38 The Determination of Phosphorylation Sites in Peptides and Proteins by Mass Spectrometry
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    Chapter 39 Identification of Phosphorylated Amino Acid Residues During Gas Phase Sequencing
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    Chapter 40 Mass Spectrometric Characterization of Microscale Enzyme Catalyzed Reactions of Surface-Bound Peptides and Proteins
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    Chapter 41 Identification of Posttranslational Modifications in Neuropeptides
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    Chapter 42 Reinventing the Wheel: General Approaches to the Elucidation of Blocked N-Terminal Sequences
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    Chapter 43 The Use of Site-Directed Mutagenesis to Study Disulphide Bond Formation and Posttranslational Processing of Insulin Precursors Expressed and Secreted from Yeast
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    Chapter 44 Sensitivity and Selectivity in Protein Sequence Comparison
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    Chapter 45 Structure and Activity-Regulation of Chimeric Proteins a Case Study of Calcium-Activated Neutral Protease
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    Chapter 46 The Receptor-Binding Sequence of Urokinase
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    Chapter 47 Identification and Interpretation of Potential Domains in Protein Sequences
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    Chapter 48 Strategies for Structural Analysis of Large Proteins: The Utility of Domains, Peptide Sequences, and Peptide Mapping
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    Chapter 49 Hydrophobicity Profiles Revisited
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    Chapter 50 Determination of Secondary and Tertiary Structures of Proteins in Solution by Two-Dimensional NMR
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    Chapter 51 Improved Hydrophilicity Plotting Method for Membrane Proteins
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    Chapter 52 The Structural Characterization of Protein-Protein Crosslinks in Ribosomes of Different Organisms
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    Chapter 53 Carboxyl Group Reactivity in Actin
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    Chapter 54 Interactions of Hydroxyboron Compounds with Amino Acids and Proteins
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    Chapter 55 Concluding Remarks