Chapter title |
Kinetics of Motor Protein Translocation on Single Stranded DNA
|
---|---|
Chapter number | 4 |
Book title |
Helicases
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-60327-355-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-60327-354-1, 978-1-60327-355-8
|
Authors |
Christopher J. Fischer, Lake Wooten, Eric J. Tomko, Timothy M. Lohman, Fischer, Christopher J., Wooten, Lake, Tomko, Eric J., Lohman, Timothy M. |
Abstract |
The translocation of nucleic acid motor proteins along DNA or RNA can be studied in ensemble experiments by monitoring either the kinetics of the arrival of the protein at a specific site on the nucleic acid filament (generally one end of the filament) or the kinetics of ATP hydrolysis by the motor protein during translocation. The pre-steady state kinetic data collected in ensemble experiments can be analyzed by simultaneous global non-linear least squares (NLLS) analysis using a simple sequential "n-step" mechanism to obtain estimates of the rate-limiting step(s) in the translocation cycle, the average "kinetic step-size," and the efficiency of coupling ATP binding and hydrolysis to translocation. |
Mendeley readers
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Demographic breakdown
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Student > Doctoral Student | 4 | 17% |
Researcher | 4 | 17% |
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Professor | 2 | 9% |
Other | 3 | 13% |
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Chemistry | 1 | 4% |
Other | 0 | 0% |
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