| Chapter title |
Strategies for the isolation and characterization of antibacterial lantibiotics.
|
|---|---|
| Chapter number | 3 |
| Book title |
Antimicrobial Peptides
|
| Published in |
Methods in molecular biology, January 2010
|
| DOI | 10.1007/978-1-60761-594-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-60761-593-4, 978-1-60761-594-1
|
| Authors |
Daniela Jabes, Stefano Donadio, Jabes, Daniela, Donadio, Stefano |
| Abstract |
Lantibiotics are biologically active peptides produced by several strains from the phyla Firmicutes and Actinobacteria. They are ribosomally synthesized and undergo posttranslational modifications that endow them with the characteristic (methyl)-lanthionine residues. As a result, lantibiotics contain a variable number of rings, each carrying one thioether link. Many lantibiotics inhibit growth of Gram-positive bacterial strains by interfering with peptidoglycan formation. Because they bind to the key intermediate lipid II at a site not affected by clinically used antibiotics, they are effective against multidrug-resistant strains. We describe a bioassay-based method suitable for finding antibacterial lantibiotics from actinomycete strains and provide selected procedures for characterizing newly discovered lantibiotics for their antibacterial properties. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 4% |
| Unknown | 26 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 30% |
| Student > Master | 5 | 19% |
| Researcher | 4 | 15% |
| Student > Doctoral Student | 3 | 11% |
| Other | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 26% |
| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Chemistry | 3 | 11% |
| Chemical Engineering | 2 | 7% |
| Business, Management and Accounting | 1 | 4% |
| Other | 4 | 15% |
| Unknown | 7 | 26% |