Chapter title |
Minisatellite variant repeat mapping: application to DNA typing and mutation analysis.
|
---|---|
Chapter number | 12 |
Book title |
DNA Fingerprinting: State of the Science
|
Published in |
EXS, January 1993
|
DOI | 10.1007/978-3-0348-8583-6_12 |
Pubmed ID | |
Book ISBNs |
978-3-76-432906-8, 978-3-03-488583-6
|
Authors |
Jeffreys, A J, Monckton, D G, Tamaki, K, Neil, D L, Armour, J A, MacLeod, A, Collick, A, Allen, M, Jobling, M, Jeffreys, A. J., Monckton, D. G., Tamaki, K., Neil, D. L., Armour, J. A. L., MacLeod, A., Collick, A., Allen, M., Jobling, M. |
Abstract |
Most DNA typing systems assay allele length variation at tandemly repeated loci such as minisatellites and microsatellites. Allele length measurements are approximate, which impedes the use of such loci in forensic analysis and in studies of allelic variability at hypervariable loci. We now review progress in the development of alternative DNA typing systems based on allelic variation in the interspersion patterns of variant repeat units along minisatellite alleles. Minisatellite variant repeat mapping by PCR (MVR-PCR) not only provides a powerful new digital approach to DNA typing, but also for the first time allows investigation of the true level of allelic variability at minisatellite loci and of the mutational mechanisms that generate ultravariability. |
Mendeley readers
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Demographic breakdown
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Unspecified | 1 | 11% |
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