Chapter title |
Probing the reactivity of the zinc and cadmium ions bound to rabbit liver metallothioneins with EDTA.
|
---|---|
Chapter number | 12 |
Book title |
Metallothionein II
|
Published in |
Experientia Supplementum, January 1987
|
DOI | 10.1007/978-3-0348-6784-9_12 |
Pubmed ID | |
Book ISBNs |
978-3-03-486786-3, 978-3-03-486784-9
|
Authors |
J. K. Nicholson, Peter J. Sadler, M. Vašák, Nicholson, J. K., Sadler, Peter J., Vašák, M. |
Abstract |
Reactions of native and reconstituted rabbit liver metallothioneins (MT's) with excess EDTA at pH 7 were studied by 1H NMR, electronic absorption and atomic absorption spectroscopy. There was a reasonable agreement between the amounts of Zn2+ and Cd2+ removed as determined by the various methods. Specific structural changes accompanying metal removal were observable by NMR. Most of the Zn2+ in Zn7-MT, reacted rapidly with EDTA. One to two g-ions of Zn2+ in native Cd,Zn-MT's were labile, but none of the Cd2+ was extracted rapidly by EDTA. The cadmium protein Cd7-MT1, reconstituted via the low pH-apothionein method, appeared to contain EDTA-reactive Cd2+ bound in a different manner to either Zn2+ or Cd2+ in the native protein. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Professor | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Student > Master | 1 | 17% |
Other | 1 | 17% |
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Arts and Humanities | 1 | 17% |
Unknown | 1 | 17% |