PCR Primer Design

Chapter 1 Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes

Chapter 2 Primer Design for PCR Reactions in Forensic Biology

Chapter 3 Design of Primers and Probes for Quantitative Real-Time PCR Methods

Chapter 4 Large-Scale Nucleotide Sequence Alignment and Sequence Variability Assessment to Identify the Evolutionarily Highly Conserved Regions for Universal Screening PCR Assay Design: An Example of Influenza A Virus

Chapter 5 Low-Concentration Initiator Primers Improve the Amplification of Gene Targets with High Sequence Variability

Chapter 6 Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens

Chapter 7 Degenerate Primer Design for Highly Variable Genomes

Chapter 8 Allele-Specific Real-Time Polymerase Chain Reaction as a Tool for Urate Transporter 1 Mutation Detection

Chapter 9 MultiPLX: Automatic Grouping and Evaluation of PCR Primers

Chapter 10 In Silico PCR Primer Designing and Validation

Chapter 11 Primer Design Using Primer Express ® for SYBR Green-Based Quantitative PCR

Chapter 12 Designing Primers for SNaPshot Technique

Chapter 14 PRIMEGENSw3: A Web-Based Tool for High-Throughput Primer and Probe Design.

Chapter 15 Selecting Specific PCR Primers with MFEprimer