Principles and Technical Aspects of PCR Amplification

Chapter 2 A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification

Chapter 3 The PCR in Practice

Chapter 4 The Different Types and Varieties of Nucleic Acid Target Molecules

Chapter 5 PCR Primers

Chapter 6 Deoxynucleotide Triphosphates and Buffer Components

Chapter 7 Principles and Technical Aspects of PCR Amplification

Chapter 8 Important Considerations for Typical, Quantitative and Real-Time PCR Protocols

Chapter 9 Analysis of PCR Amplification Products

Chapter 10 Ensuring PCR Quality – Laboratory Organisation, PCR Optimization and Controls

Chapter 11 Ensuring PCR Quality – Quality Criteria and Quality Assurance

Chapter 12 Variants and Adaptations of the Standard PCR Protocol

Chapter 13 In Situ PCR Amplification (ISA) – Major Considerations, Sample Processing and Applications