| Chapter title |
Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
|
|---|---|
| Chapter number | 16 |
| Book title |
RNA-Protein Complexes and Interactions
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3591-8_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3589-5, 978-1-4939-3591-8
|
| Authors |
Zhou, Jiehua, Rossi, John J., Jiehua Zhou, John J. Rossi |
| Abstract |
Live cell-based SELEX (Systematic Evolution of Ligand EXponential enrichment) is a promising approach for identifying aptamers that can selectively bind to a cell-surface antigen or a particular target cell population. In particular, it offers a facile selection strategy for some special cell-surface proteins that are original glycosylated or heavily post-translationally modified, and are unavailable in their native/active conformation after in vitro expression and purification. In this chapter, we describe evolution of cell-type-specific RNA aptamers targeting the human CCR5 by combining the live cell-based SELEX strategy with high-throughput sequencing (HTS) and bioinformatics analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 40% |
| Student > Ph. D. Student | 1 | 20% |
| Unknown | 2 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 1 | 20% |
| Neuroscience | 1 | 20% |
| Unknown | 3 | 60% |