Chapter title |
Evaluating Chemical CDK Inhibitors as Cell Death Inducers
|
---|---|
Chapter number | 14 |
Book title |
Cyclin-Dependent Kinase (CDK) Inhibitors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2926-9_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2925-2, 978-1-4939-2926-9
|
Authors |
Hiroshi Hirai, Yoko Nakatsuru |
Abstract |
The cell cycle of eukaryotic cells is regulated by a family of protein kinases called cyclin-dependent kinases (Cdks). We have reported the identification and biological characterization of a highly potent, small-molecule pan-Cdk inhibitor, which inhibited Cdk1, 2, 4, 5, 6, and 9 with equal potency in the nM range. This compound inhibited multiple events in the cell cycle and induced cell death in human cancer cell lines as well as in peripheral blood or purified resting lymphocytes ex vivo. We describe the materials and methods to determine antitumor efficacy in vivo xenograft models. Pharmacodynamic marker assays that have been performed using tumors and normal tissues are explained. Moreover, we briefly describe methods for determining the effects of chemical Cdk inhibitors on peripheral blood cells or lymphocytes ex vivo. |
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