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Mendeley readers
Chapter title |
Virus-Induced Gene Silencing as a Tool to Study Tomato Fruit Biochemistry
|
---|---|
Chapter number | 7 |
Book title |
Plant Signal Transduction
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3115-6_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3114-9, 978-1-4939-3115-6
|
Authors |
Elio Fantini, Giovanni Giuliano, Fantini, Elio, Giuliano, Giovanni |
Abstract |
Virus-Induced Gene Silencing (VIGS) is an excellent reverse genetic tool for the study of gene function in plants, based on virus infection. In this chapter, we describe a high-throughput approach based on VIGS for the study of tomato fruit biochemistry. It comprises the selection of the sequence for silencing using bioinformatics tools, the cloning of the fragment in the Tobacco Rattle Virus (TRV), and the agroinfiltration of tomato fruits mediated by Agrobacterium tumefaciens. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 22% |
Student > Doctoral Student | 3 | 17% |
Researcher | 2 | 11% |
Student > Master | 2 | 11% |
Student > Bachelor | 1 | 6% |
Other | 3 | 17% |
Unknown | 3 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 56% |
Biochemistry, Genetics and Molecular Biology | 3 | 17% |
Chemical Engineering | 1 | 6% |
Unknown | 4 | 22% |