| Chapter title |
SuperSAGE as an Analytical Tool for Host and Viral Gene Expression.
|
|---|---|
| Chapter number | 14 |
| Book title |
Plant Virology Protocols
|
| Published in |
Methods in molecular biology, October 2014
|
| DOI | 10.1007/978-1-4939-1743-3_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1742-6, 978-1-4939-1743-3
|
| Authors |
Matsumura H, Krüger DH, Kahl G, Terauchi R, Hideo Matsumura, Detlev H. Krüger, Günter Kahl, Ryohei Terauchi, Matsumura, Hideo, Krüger, Detlev H., Kahl, Günter, Terauchi, Ryohei |
| Abstract |
SuperSAGE is a tag-based transcript profiling method, which allows to analyze the expression of thousands of genes at a time. In SuperSAGE, 26 bp tags are extracted from cDNA using the type III restriction enzyme, EcoP15I. In SuperSAGE, the amount of transcripts was represented by tag counts. Taking advantage of uniqueness of the 26 bp tags, host and virus transcripts can be monitored in virus-infected cells. Combining next generation sequencing technology, we established High-throughput SuperSAGE (Ht-SuperSAGE), which allows the analysis of multiple samples with reduced time and cost. In this chapter, we present the protocol of Ht-SuperSAGE involving a recently available benchtop type next generation sequencer. |
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