Chapter title |
Analysis of neutrophil bactericidal activity.
|
---|---|
Chapter number | 19 |
Book title |
Neutrophil Methods and Protocols
|
Published in |
Methods in molecular biology, February 2014
|
DOI | 10.1007/978-1-62703-845-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-62703-844-7, 978-1-62703-845-4
|
Authors |
Parker HA, Magon NJ, Green JN, Hampton MB, Winterbourn CC, Heather A. Parker, Nicholas J. Magon, Jessie N. Green, Mark B. Hampton, Christine C. Winterbourn, Parker, Heather A., Magon, Nicholas J., Green, Jessie N., Hampton, Mark B., Winterbourn, Christine C. |
Abstract |
This chapter describes two methods for measuring the bactericidal activity of neutrophils. These are a new simple fluorescence-based assay, which quantifies bactericidal activity by measuring changes in bacterial fluorescence associated with a loss of membrane potential over time, and a more traditional colony counting protocol. Two variations of these techniques are presented: a "one-step" protocol providing a composite measure of phagocytosis and killing, and a "two-step" protocol that allows calculation of separate rate constants for both of these processes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 30% |
Professor | 2 | 20% |
Researcher | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 40% |
Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Social Sciences | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Unknown | 1 | 10% |