Chapter title |
Flow cytometric methods for sperm assessment.
|
---|---|
Chapter number | 16 |
Book title |
Spermatogenesis
|
Published in |
Methods in molecular biology, September 2012
|
DOI | 10.1007/978-1-62703-038-0_16 |
Pubmed ID | |
Book ISBNs |
978-1-62703-037-3, 978-1-62703-038-0
|
Authors |
Robles V, Martínez-Pastor F, Vanesa Robles, Felipe Martínez-Pastor, Robles, Vanesa, Martínez-Pastor, Felipe |
Abstract |
Flow cytometry allows the assessment of multiple sperm parameters following a diverse number of protocols. Here, we describe three methods for evaluating critical aspects of sperm quality: the double stain SYBR-14 and propidium iodide (PI) for assessing sperm viability; the double stain PNA-FITC (peanut agglutinin-fluorescein isothiocyanate) and PI for assessing acrosomal integrity combined with sperm viability; and JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolylcarbocyanine iodide) for assessing mitochondrial membrane potential. These three stains are widely used and can be analyzed with basic flow cytometers. |
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