Chapter title |
3D Super-Resolution Imaging by Localization Microscopy
|
---|---|
Chapter number | 11 |
Book title |
Methods in Membrane Lipids
|
Published in |
Methods in molecular biology, September 2014
|
DOI | 10.1007/978-1-4939-1752-5_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1751-8, 978-1-4939-1752-5
|
Authors |
Magenau, Astrid, Gaus, Katharina, Astrid Magenau, Katharina Gaus |
Abstract |
Fluorescence microscopy is an important tool in all fields of biology to visualize structures and monitor dynamic processes and distributions. Contrary to conventional microscopy techniques such as confocal microscopy, which are limited by their spatial resolution, super-resolution techniques such as photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) have made it possible to observe and quantify structure and processes on the single molecule level. Here, we describe a method to image and quantify the molecular distribution of membrane-associated proteins in two and three dimensions with nanometer resolution. |
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