Chapter title |
Chromatin imaging with time-lapse atomic force microscopy.
|
---|---|
Chapter number | 3 |
Book title |
Chromatin Protocols
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2474-5_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2473-8, 978-1-4939-2474-5
|
Authors |
Lyubchenko, Yuri L, Shlyakhtenko, Luda S, Yuri L. Lyubchenko, Luda S. Shlyakhtenko, Lyubchenko, Yuri L., Shlyakhtenko, Luda S. |
Abstract |
Time-lapse atomic force microscopy (AFM) is widely used for direct visualization of the nanoscale dynamics of various biological systems. The advent of high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the high-speed AFM instrument. The necessary specifics for the preparation of chromatin samples for imaging with AFM including the protocols for the surface preparation are provided. |
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Materials Science | 1 | 7% |
Other | 0 | 0% |
Unknown | 2 | 14% |