Chapter title |
High-Dimensional Analysis of Human CD8(+) T Cell Phenotype, Function, and Antigen Specificity.
|
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Chapter number | 354 |
Book title |
High-Dimensional Single Cell Analysis
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Published in |
Current topics in microbiology and immunology, October 2013
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DOI | 10.1007/82_2013_354 |
Pubmed ID | |
Book ISBNs |
978-3-64-254826-0, 978-3-64-254827-7
|
Authors |
Evan W Newell, Wenyu Lin, Evan W. Newell, Newell, Evan W., Lin, Wenyu |
Abstract |
Antigen-specific T cells are critical initiators and orchestrators of the adaptive immune response. Categorizing antigen-specific T cell subsets is not a simple task given the diversity of these cells and the large number of parameters that can be considered. Here, we focus on human CD8(+) T cells and discuss the utility of high-dimensional mass cytometric analysis techniques for the concurrent identification and characterization of antigen-specific T cells involved in immunological homeostasis and disease. We first provide an overview of previously identified T cell subsets. We then discuss the segregation of antigen-specific T cells based on protein expression through surface and/or intracellular staining, on functional capacity through measurement expression of cytokines or other inducible markers, and on the antigen-specificity of the cell assessed using peptide-major histocompatibility complex multimers. High-dimensional mass cytometry enables a deeper and more integrated view of all three aspects of antigen-specific T cell diversity than do traditional techniques. Use of mass cytometry for precise measurement of the status of antigen-specific immune responses should result in better prediction of vaccine efficacy and disease outcomes. |
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