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Guidelines for Molecular Analysis in Archive Tissues

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Cover of 'Guidelines for Molecular Analysis in Archive Tissues'

Table of Contents

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    Book Overview
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    Chapter 1 Archive Tissues
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    Chapter 2 Preanalytical Time Interval (PATI) and Fixation
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    Chapter 3 Formalin-Free Fixatives
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    Chapter 4 Manual Microdissection
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    Chapter 5 Tissue Microarray (TMA)
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    Chapter 6 Laser Capture Microdissection (LCM)
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    Chapter 7 DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues
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    Chapter 8 DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells)
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    Chapter 9 Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues
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    Chapter 10 DNA Extraction from Blood and Forensic Samples
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    Chapter 11 Restoration and Reconstruction of DNA Length
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    Chapter 12 RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues
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    Chapter 13 RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues
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    Chapter 14 RNA Temperature Demodification
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    Chapter 15 MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues
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    Chapter 16 Quantification of Nucleic Acids
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    Chapter 17 Integrity Assessment of Nucleic Acids
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    Chapter 18 DNase Treatment of RNA
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    Chapter 19 Reverse Transcription
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    Chapter 20 General Protocol for End-Point PCR
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    Chapter 21 Quantitative End-Point PCR
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    Chapter 22 Nested-PCR
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    Chapter 23 General Protocol for Dot-Blot
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    Chapter 24 PCR-ELISA
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    Chapter 25 Quantitative Real-Time RT-PCR
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    Chapter 26 DNA Sequencing from PCR Products
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    Chapter 27 Pyrosequencing Analysis for Detection of KRAS Mutation
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    Chapter 28 Microsatellite Instability (MSI) Detection in DNA from FFPE Tissues
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    Chapter 29 General Protocol for Loss of Heterozygosity Detection
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    Chapter 30 Qualitative Methylation Status Assessment
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    Chapter 31 Quantitative Methylation Status Assessment in DNA from FFPE Tissues with Bisulfite Modification and Real-Time Quantitative MSP
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    Chapter 32 Comparative Genomic Hybridisation (CGH)
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    Chapter 33 Multiplex Ligation-dependent Probe Amplification (MLPA)
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    Chapter 34 Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections
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    Chapter 35 Introduction on Genome-wide Expression Profiling from Formalin-Fixed Paraffin-Embedded Tissues Using Microarrays
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    Chapter 36 Introduction
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    Chapter 37 Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissues
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    Chapter 38 Rapid Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue Samples
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    Chapter 39 Protein Extraction Protocol from Tissues Treated with Formalin-Free Fixatives
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    Chapter 40 Trichloroacetic Acid (TCA) Precipitation of Proteins
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    Chapter 41 One-Dimensional Sodium-Dodecyl-Sulfate (SDS) Polyacrylamide Gel Electrophoresis
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    Chapter 42 Visualization of Proteins
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    Chapter 43 Western Blotting
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    Chapter 44 Dot Blot
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    Chapter 45 Membrane Stripping
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    Chapter 46 Reverse-Phase Protein Microarrays
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    Chapter 47 Introduction
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    Chapter 48 Sample Preparation and In Gel Tryptic Digestion for Mass Spectrometry Experiments
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    Chapter 49 MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissues
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    Chapter 50 Introduction
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    Chapter 51 Internal Quality Control (IQC) Organization
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    Chapter 52 Standards for Equipment and Reagents
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Title
Guidelines for Molecular Analysis in Archive Tissues
Published by
Springer Berlin Heidelberg, June 2011
DOI 10.1007/978-3-642-17890-0
ISBNs
978-3-64-217889-4, 978-3-64-217890-0
Editors

Stanta, Giorgio

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Russia 1 1%
Unknown 85 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 14 16%
Student > Ph. D. Student 11 13%
Student > Master 11 13%
Other 7 8%
Professor 5 6%
Other 12 14%
Unknown 26 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 19%
Biochemistry, Genetics and Molecular Biology 13 15%
Medicine and Dentistry 11 13%
Chemistry 3 3%
Nursing and Health Professions 2 2%
Other 12 14%
Unknown 29 34%