Chapter title |
Mining Exosomal MicroRNAs from Human-Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration
|
---|---|
Chapter number | 10 |
Book title |
MicroRNA Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7601-0_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7600-3, 978-1-4939-7601-0
|
Authors |
Sang-Ging Ong, Won Hee Lee, Yang Zhou, Joseph C. Wu, Ong, Sang-Ging, Lee, Won Hee, Zhou, Yang, Wu, Joseph C. |
Abstract |
Myocardial infarction is the leading cause of morbidity and mortality worldwide. Recent advances in cardiac regenerative therapy have allowed for novel modalities in replenishing the damaged myocardium. However, poor long-term engraftment and survival of transplanted cells have largely precluded effective cell replacement. As an alternative to direct cell replacement, the release of paracrine protective factors may be a more plausible effector for cardioprotection which may partially be mediated through secretion of microvesicles, or exosomes, that contribute to cell-cell communication. In this chapter, we describe the isolation of exosomes from induced pluripotent stem cells-derived cardiomyocytes for subsequent microRNA profiling for a better understanding of the biological cargo contained within exosomes. |
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Geographical breakdown
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Unknown | 22 | 100% |
Demographic breakdown
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Researcher | 3 | 14% |
Student > Doctoral Student | 2 | 9% |
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Lecturer > Senior Lecturer | 1 | 5% |
Other | 1 | 5% |
Unknown | 8 | 36% |
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Engineering | 1 | 5% |
Other | 0 | 0% |
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