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Metastasis Research Protocols

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Cover of 'Metastasis Research Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 In Vitro Invasion Assay Using Matrigel™: A Reconstituted Basement Membrane Preparation
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    Chapter 2 Single Cell and Spheroid Collagen Type I Invasion Assay
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    Chapter 3 Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells
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    Chapter 4 Small-Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin Under Physiological Flow Conditions.
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    Chapter 5 Adhesion of Tumor Cells to Matrices and Endothelium
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    Chapter 6 Cell Aggregation Assays
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    Chapter 7 Chick Heart Invasion Assay
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    Chapter 8 Computer Simulation of the Metastatic Progression
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    Chapter 9 Theoretical Considerations in Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice
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    Chapter 10 Syngeneic Murine Metastasis Models: B16 Melanoma
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    Chapter 11 Imageable Clinically Relevant Mouse Models of Metastasis
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    Chapter 12 Imaging metastatic cell trafficking at the cellular level in vivo with fluorescent proteins.
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    Chapter 13 Ultrasound Techniques for the Detection of Tumors and Metastases in Small Animals
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    Chapter 14 The PFP/RAG2 Double-Knockout Mouse in Metastasis Research: Small-Cell Lung Cancer and Prostate Cancer.
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    Chapter 15 Ultrasound-Guided Intracardial Injection and In Vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Hematogenous Metastases.
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    Chapter 16 Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer
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    Chapter 17 Spontaneous and Experimental Metastasis Models: Nude Mice
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    Chapter 18 Identifying the Origin and Phenotype of Cells in Tumor Xenografts
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    Chapter 19 Erratum
Attention for Chapter 10: Syngeneic Murine Metastasis Models: B16 Melanoma
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Chapter title
Syngeneic Murine Metastasis Models: B16 Melanoma
Chapter number 10
Book title
Metastasis Research Protocols
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4614-8244-4_10
Pubmed ID
Book ISBNs
978-1-4614-8243-7, 978-1-4614-8244-4
Authors

Raffaella Giavazzi, Alessandra Decio

Abstract

The murine B16 melanoma is one of the most used tumor models, its application having been used to determine the mechanisms associated with the metastatic process and the development of anticancer therapies. The B16 melanoma was originally established by Fidler and collaborators as a tumor line metastasizing to the lung. Since that time a variety of cell lines have been derived, in vitro or in vivo, having different metastatic behaviors.The methods used to obtain artificial metastases to the lung through the intravenous injection of B16 melanoma cells and spontaneous metastasis formation following cancer cell growth in the footpad are described in this chapter.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 57 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 30%
Student > Ph. D. Student 12 21%
Student > Master 8 14%
Student > Bachelor 2 4%
Unspecified 2 4%
Other 7 12%
Unknown 9 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 23%
Agricultural and Biological Sciences 11 19%
Pharmacology, Toxicology and Pharmaceutical Science 7 12%
Immunology and Microbiology 7 12%
Medicine and Dentistry 3 5%
Other 5 9%
Unknown 11 19%