Chapter title |
Methods to Immobilize GPCR on the Surface of SPR Sensors.
|
---|---|
Chapter number | 12 |
Book title |
G Protein-Coupled Receptor Screening Assays
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2336-6_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2335-9, 978-1-4939-2336-6
|
Authors |
Laura Martínez-Muñoz, Rubén Barroso, Anabel Guedán Paredes, Mario Mellado, José Miguel Rodríguez-Frade, Martínez-Muñoz, Laura, Barroso, Rubén, Paredes, Anabel Guedán, Mellado, Mario, Rodríguez-Frade, José Miguel |
Abstract |
The G protein-coupled receptors (GPCRs) form one of the largest membrane receptor families. The nature of the ligands that interact with these receptors is highly diverse; they include light, peptides and hormones, neurotransmitters, and small molecular weight compounds. The GPCRs are involved in a wide variety of physiological processes and thus hold considerable therapeutic potential.GPCR function is usually determined in cell-based assays, whose complexity nonetheless limits their use. The use of alternative, cell-free assays is hampered by the difficulties in purifying these seven-transmembrane domain receptors without altering their functional properties. Several methods have been proposed to immobilize GPCR on biosensor surfaces which use antibodies or avidin-/biotin-based capture procedures, alone or with reconstitution of the GPCR physiological microenvironment. Here we propose a method for GPCR immobilization in their native membrane microenvironment that requires no manipulation of the target receptor and maintains the many conformations GPCR can adopt in the cell membrane. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 7% |
Unknown | 13 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 29% |
Student > Ph. D. Student | 3 | 21% |
Student > Master | 2 | 14% |
Student > Bachelor | 1 | 7% |
Other | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 36% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Engineering | 2 | 14% |
Medicine and Dentistry | 1 | 7% |
Chemistry | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 14% |