Chapter title |
Immunofluorescence detection of callose deposition around plasmodesmata sites.
|
---|---|
Chapter number | 6 |
Book title |
Plasmodesmata
|
Published in |
Methods in molecular biology, October 2014
|
DOI | 10.1007/978-1-4939-1523-1_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1522-4, 978-1-4939-1523-1
|
Authors |
Pendle A, Benitez-Alfonso Y, Ali Pendle, Yoselin Benitez-Alfonso, Pendle, Ali, Benitez-Alfonso, Yoselin |
Abstract |
Accumulation of callose (β-1,3 glucans) at the plasmodesmata (PD) neck region dynamically regulates symplastic intercellular transport. Here we describe a 2-3-day immuno-labelling protocol to determine callose levels in the cell wall region at PD. The method relies on exposure of internal cell walls by hand-sectioning of the sample and digestion of the cell wall with enzymes in order to improve antibody penetration to deep tissue layers. By using this protocol, combined with high-resolution confocal imaging, we successfully detected PD-associated callose in Arabidopsis root apical meristem, vascular tissue, and developing lateral root primordia. |
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