Chapter title |
Role of protein kinase C and phosphatases in 12(S)-HETE-induced tumor cell cytoskeletal reorganization.
|
---|---|
Chapter number | 48 |
Book title |
Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury 2
|
Published in |
Advances in experimental medicine and biology, January 1997
|
DOI | 10.1007/978-1-4615-5325-0_48 |
Pubmed ID | |
Book ISBNs |
978-1-4613-7430-5, 978-1-4615-5325-0
|
Authors |
Dean G. Tang, Kenneth V. Honn, Tang, Dean G., Honn, Kenneth V. |
Abstract |
Adherent B16 amelanotic melanoma (B16a) cells exposed to fatty acid 12(S)-HETE, a lipoxygenase metabolite of arachidonic acid, demonstrated a gradual dissolution of stress fibers and bundling-together of vimentin. The 12(S)-HETE effects on tumor cell cytoskeleton appeared 5 min after treatment, became prominent approximately 15 min following stimulation, and generally disappeared by 30 min. Simultaneous treatment of cells with 12(S)-HETE and okadaic acid (OA) prevented disappearance of the 12(S)-HETE effects by 30 min. Quantitative double immunoblotting of actin and vimentin indicated that actin, but not vimentin, underwent a time-related depolymerization. On the other hand, enhanced phosphorylation of vimentin but not of actin was observed after 12(S)-HETE treatment. 12(S)-HETE-enhanced vimentin phosphorylation was abolished by protein kinase C (PKC) inhibitor calphostin C, thus suggesting the involvement of PKC. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 2 | 29% |
Student > Ph. D. Student | 1 | 14% |
Student > Bachelor | 1 | 14% |
Student > Master | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 2 | 29% |
Agricultural and Biological Sciences | 1 | 14% |
Sports and Recreations | 1 | 14% |
Medicine and Dentistry | 1 | 14% |
Unknown | 2 | 29% |