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Single-Stranded DNA Binding Proteins

Overview of attention for book
Cover of 'Single-Stranded DNA Binding Proteins'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Functions of Single-Strand DNA-Binding Proteins in DNA Replication, Recombination, and Repair
  3. Altmetric Badge
    Chapter 2 Structural Diversity Based on Variability in Quaternary Association. A Case Study Involving Eubacterial and Related SSBs
  4. Altmetric Badge
    Chapter 3 SSB Binding to ssDNA Using Isothermal Titration Calorimetry.
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    Chapter 4 SSB–DNA Binding Monitored by Fluorescence Intensity and Anisotropy
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    Chapter 5 Single-Molecule Analysis of SSB Dynamics on Single-Stranded DNA
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    Chapter 6 Sample Preparation Methods to Analyze DNA-Induced Structural Changes in Replication Protein A.
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    Chapter 7 Structural Studies of SSB Interaction with RecO
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    Chapter 8 Investigation of Protein–Protein Interactions of Single-Stranded DNA-Binding Proteins by Analytical Ultracentrifugation
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    Chapter 9 Ammonium Sulfate Co-precipitation of SSB and Interacting Proteins
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    Chapter 10 Analyzing Interactions Between SSB and Proteins by the Use of Fluorescence Anisotropy
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    Chapter 11 Far Western Blotting as a Rapid and Efficient Method for Detecting Interactions Between DNA Replication and DNA Repair Proteins
  13. Altmetric Badge
    Chapter 12 Methods for Analysis of SSB–Protein Interactions by SPR
  14. Altmetric Badge
    Chapter 13 Use of Native Gels to Measure Protein Binding to SSB
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    Chapter 14 Identification of Small Molecules That Disrupt SSB–Protein Interactions Using a High-Throughput Screen
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    Chapter 15 Detection of Posttranslational Modifications of Replication Protein A
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    Chapter 16 Detecting Posttranslational Modifications of Bacterial SSB Proteins
  18. Altmetric Badge
    Chapter 17 Fluorescent SSB as a Reagentless Biosensor for Single-Stranded DNA
  19. Altmetric Badge
    Chapter 18 Fluorescent Single-Stranded DNA-Binding Proteins Enable In Vitro and In Vivo Studies
  20. Altmetric Badge
    Chapter 19 Use of Fluorescently Tagged SSB Proteins in In Vivo Localization Experiments
Attention for Chapter 3: SSB Binding to ssDNA Using Isothermal Titration Calorimetry.
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Chapter title
SSB Binding to ssDNA Using Isothermal Titration Calorimetry.
Chapter number 3
Book title
Single-Stranded DNA Binding Proteins
Published in
Methods in molecular biology, September 2012
DOI 10.1007/978-1-62703-032-8_3
Pubmed ID
22976176
Book ISBNs
978-1-62703-031-1, 978-1-62703-032-8
Authors

Kozlov AG, Lohman TM, Alexander G. Kozlov, Timothy M. Lohman, Kozlov, Alexander G., Lohman, Timothy M.

Abstract

Isothermal titration calorimetry (ITC) is a powerful method for studying protein-DNA interactions in solution. As long as binding is accompanied by an appreciable enthalpy change, ITC studies can yield quantitative information on stoichiometries, binding energetics (affinity, binding enthalpy and entropy) and potential site-site interactions (cooperativity). This can provide a full thermodynamic description of an interacting system which is necessary to understand the stability and specificity of protein-DNA interactions and to correlate the activities or functions of different species. Here we describe procedures to perform and analyze ITC studies using as examples, the E. coli SSB (homotetramer with 4 OB-folds) and D. radiodurans SSB (homodimer with 4 OB-folds). For oligomeric protein systems such as these, we emphasize the need to be aware of the likelihood that solution conditions will influence not only the affinity and enthalpy of binding but also the mode by which the SSB oligomer binds ssDNA.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 39%
Researcher 3 17%
Professor 2 11%
Student > Doctoral Student 1 6%
Student > Bachelor 1 6%
Other 2 11%
Unknown 2 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 50%
Agricultural and Biological Sciences 4 22%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Physics and Astronomy 1 6%
Chemistry 1 6%
Other 0 0%
Unknown 2 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2014.
All research outputs
#18,369,403
of 22,751,628 outputs
Outputs from Methods in molecular biology
#7,861
of 13,089 outputs
Outputs of similar age
#115,515
of 152,116 outputs
Outputs of similar age from Methods in molecular biology
#37
of 70 outputs
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So far Altmetric has tracked 13,089 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 70 others from the same source and published within six weeks on either side of this one. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.

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