Chapter title |
MHV-3 induced prothrombinase is encoded by musfiblp.
|
---|---|
Chapter number | 25 |
Book title |
Corona- and Related Viruses
|
Published in |
Advances in experimental medicine and biology, January 1995
|
DOI | 10.1007/978-1-4615-1899-0_25 |
Pubmed ID | |
Book ISBNs |
978-1-4613-5775-9, 978-1-4615-1899-0
|
Authors |
Parr, R L, Fung, L, Reneker, J, Myers-Mason, N, Leibowitz, J L, Levy, G, Rebecca L. Parr, Laisum Fung, Jeffrey Reneker, Nancy Myers-Mason, Julian L. Leibowitz, Gary Levy, Parr, Rebecca L., Fung, Laisum, Reneker, Jeffrey, Myers-Mason, Nancy, Leibowitz, Julian L., Levy, Gary |
Abstract |
Previously, we demonstrated induction of a unique macrophage prothrombinase, PCA, in MHV-3 infected BALB/cJ mice. By immunologic screening, a clone representing PCA was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp, but when infected with MHV-3, synthesized musfiblp-specific mRNA. Musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrate when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 33% |
Student > Bachelor | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |
Unknown | 1 | 33% |