Chapter title |
Combination of theoretical and experimental approaches for the design and study of fibril-forming peptides.
|
---|---|
Chapter number | 3 |
Book title |
Protein Design
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1486-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1485-2, 978-1-4939-1486-9
|
Authors |
Phanourios Tamamis, Emmanouil Kasotakis, Georgios Archontis, Anna Mitraki |
Abstract |
Self-assembling peptides that can form supramolecular structures such as fibrils, ribbons, and nanotubes are of particular interest to modern bionanotechnology and materials science. Their ability to form biocompatible nanostructures under mild conditions through non-covalent interactions offers a big biofabrication advantage. Structural motifs extracted from natural proteins are an important source of inspiration for the rational design of such peptides. Examples include designer self-assembling peptides that correspond to natural coiled-coil motifs, amyloid-forming proteins, and natural fibrous proteins. In this chapter, we focus on the exploitation of structural information from beta-structured natural fibers. We review a case study of short peptides that correspond to sequences from the adenovirus fiber shaft. We describe both theoretical methods for the study of their self-assembly potential and basic experimental protocols for the assessment of fibril-forming assembly. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 18% |
Student > Master | 2 | 18% |
Researcher | 2 | 18% |
Professor > Associate Professor | 1 | 9% |
Student > Postgraduate | 1 | 9% |
Other | 0 | 0% |
Unknown | 3 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Materials Science | 2 | 18% |
Agricultural and Biological Sciences | 1 | 9% |
Engineering | 1 | 9% |
Unknown | 4 | 36% |