| Chapter title |
Purification of the Outer Membrane Usher Protein and Periplasmic Chaperone-Subunit Complexes from the P and Type 1 Pilus Systems
|
|---|---|
| Chapter number | 3 |
| Book title |
Bacterial Cell Surfaces
|
| Published in |
Methods in molecular biology, January 2013
|
| DOI | 10.1007/978-1-62703-245-2_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-244-5, 978-1-62703-245-2
|
| Authors |
Nadine S. Henderson, David G. Thanassi, Henderson NS, Thanassi DG, Henderson, Nadine S., Thanassi, David G. |
| Abstract |
Understanding molecular mechanisms of protein secretion by bacteria requires the purification of secretion machinery components and the isolation of complexes between the secretion machinery and substrate proteins. Here, we describe methods for the purification of proteins from the chaperone/usher pathway, which is a conserved secretion pathway dedicated to the assembly of polymeric surface fibers termed pili or fimbriae in gram-negative bacteria. Specifically, we describe the isolation of the PapC and FimD usher proteins from the bacterial outer membrane, and the purification of PapD-PapG and FimC-FimH chaperone--subunit complexes from the periplasm. These Pap and Fim proteins belong to the P and type 1 pilus systems of uropathogenic Escherichia coli, respectively. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 33% |
| Student > Doctoral Student | 2 | 22% |
| Professor | 1 | 11% |
| Student > Bachelor | 1 | 11% |
| Student > Ph. D. Student | 1 | 11% |
| Other | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 44% |
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
| Immunology and Microbiology | 1 | 11% |
| Chemistry | 1 | 11% |
| Other | 0 | 0% |