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Chapter title |
RNA-Protein Interaction Protocols
|
---|---|
Chapter number | 12 |
Book title |
RNA-Protein Interaction Protocols
|
Published in |
Methods in molecular biology, January 2008
|
DOI | 10.1007/978-1-60327-475-3_12 |
Pubmed ID | |
Book ISBNs |
978-1-58829-419-7, 978-1-60327-475-3
|
Authors |
Banerjee, Hiren, Singh, Ravinder, Hiren Banerjee, Ravinder Singh |
Abstract |
A large number of proteins contain multiple RNA recognition motifs (RRMs). How multiple RRMs contribute to RNA recognition in solution is, however, poorly understood. Here, we describe a simple biochemical approach called CLAMP (crosslinking and mapping of protein domain) to identify an RRM that is crosslinked to a specific nucleotide in RNA. It involves site-specific incorporation of a chromophore, photochemical RNA-protein crosslinking, and site-specific chemical cleavage of the protein. This technique is suitable for numerous other RNA binding proteins that have multiple RNA binding domains. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 44% |
Student > Ph. D. Student | 3 | 33% |
Professor > Associate Professor | 1 | 11% |
Other | 1 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 78% |
Biochemistry, Genetics and Molecular Biology | 2 | 22% |