Chapter 1 Energy Systems for ATP Regeneration in Cell-Free Protein Synthesis Reactions

Chapter 2 Continuous-Exchange Protein-Synthesizing Systems

Chapter 3 Cell-Free Production of Integral Membrane Proteins on a Preparative Scale

Chapter 4 SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression

Chapter 5 Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System

Chapter 6 Exogenous Protein Expression in Xenopus Oocytes

Chapter 7 In Vitro Translation to Study HIV Protease Activity

Chapter 8 The Protein Truncation Test in Mutation Detection and Molecular Diagnosis

Chapter 9 Creation of Novel Enantioselective Lipases by SIMPLEX

Chapter 10 In Vitro Transcription and Translation Coupled to Two-Dimensional Electrophoresis for Bacterial Proteome Analysis

Chapter 11 In Vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation, and Immunoprecipitation Analysis

Chapter 13 Production of Protein for Nuclear Magnetic Resonance Study Using the Wheat Germ Cell-Free System