Chapter title |
Methods for Elucidating the Mechanism of Action of Proline-Rich and Other Non-lytic Antimicrobial Peptides
|
---|---|
Chapter number | 20 |
Book title |
Antimicrobial Peptides
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6737-7_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6735-3, 978-1-4939-6737-7
|
Authors |
Monica Benincasa, Giulia Runti, Mario Mardirossian, Renato Gennaro, Marco Scocchi, Benincasa, Monica, Runti, Giulia, Mardirossian, Mario, Gennaro, Renato, Scocchi, Marco |
Abstract |
A distinct group of antimicrobial peptides kills bacteria by interfering with internal cellular functions and without concurrent lytic effects on cell membranes. Here we describe some methods to investigate the mechanisms of action of these antimicrobial peptides. They include assays to detect the possible temporal separation between membrane permeabilization and bacterial killing events, to assess the capacity of antimicrobial peptides to cross the bacterial membranes and reside in the cytoplasm, and later to inhibit vital cell functions such as DNA transcription and protein translation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 25% |
Lecturer | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Student > Master | 1 | 13% |
Other | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Agricultural and Biological Sciences | 2 | 25% |
Immunology and Microbiology | 1 | 13% |
Unknown | 2 | 25% |