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Mendeley readers
Chapter title |
Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB 2
|
---|---|
Chapter number | 9 |
Book title |
Protein Affinity Tags
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1034-2_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1033-5, 978-1-4939-1034-2
|
Authors |
Silvia C. Locatelli-Hoops, Alexei A. Yeliseev |
Abstract |
Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows for efficient removal of contaminating proteins, including products of proteolytic degradation of the fusion that lack either N- or C-terminal tags. Here, we describe the use of two small affinity tags, a poly-histidine tag and a Strep-tag for expression and purification of the human cannabinoid receptor CB2, an integral membrane G protein-coupled receptor. |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 5% |
Unknown | 21 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 8 | 36% |
Researcher | 5 | 23% |
Student > Master | 3 | 14% |
Other | 2 | 9% |
Student > Doctoral Student | 1 | 5% |
Other | 1 | 5% |
Unknown | 2 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 59% |
Agricultural and Biological Sciences | 3 | 14% |
Psychology | 2 | 9% |
Medicine and Dentistry | 1 | 5% |
Neuroscience | 1 | 5% |
Other | 0 | 0% |
Unknown | 2 | 9% |