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Immunochemical Protocols

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Cover of 'Immunochemical Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Production of Polyclonal Antisera
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    Chapter 2 Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen
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    Chapter 3 Production and Characterization of Antibodies Against Synthetic Peptides
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    Chapter 4 Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins
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    Chapter 5 A Simple Hollow-Fiber Bioreactor for the “In-House” Production of Monoclonal Antibodies
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    Chapter 6 Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase
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    Chapter 7 Synthesis of Peptides for Use as Immunogens
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    Chapter 8 Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens
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    Chapter 9 Purification of Glycoproteins and Their Use as Immunogens
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    Chapter 10 IgG Purification
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    Chapter 11 Purification of Monoclonal Antibodies
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    Chapter 12 Production of Bispecif ic and Trispecific F(ab) 2 and F(ab) 3 Antibody Derivatives
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    Chapter 13 Preparation of Cytotoxic Antibody—Toxin Conjugates
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    Chapter 14 Immunoaffinity Purification and Quantification of Antibody—Toxin Conjugates
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    Chapter 15 Competitive ELISA
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    Chapter 16 Epitope Mapping
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    Chapter 17 Coupling of Antibodies with Biotin
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    Chapter 18 Preparation of Avidin Conjugates
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    Chapter 19 Enhanced Chemiluminescence Immunoassay
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    Chapter 20 Nonradioactive Methods for Visualization of Protein Blots
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    Chapter 21 Colloidal Gold Staining and Immunoprobing on the Same Western Blot
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    Chapter 22 Erasure of Western Blots After Autoradiographic or Chemiluminescent Detection
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    Chapter 23 Colloidal Gold Staining and Immunodetection in 2D Protein Mapping
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    Chapter 24 Enzyme-Antienzyme Method for Immunohistochemistry
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    Chapter 25 Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies
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    Chapter 26 Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine
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    Chapter 27 Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA)
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    Chapter 28 Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates
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    Chapter 29 Preparation of Colloidal Gold Probes
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    Chapter 30 Immunogold Probes in Light Microscopy
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    Chapter 31 Immunogold Probes in Electron Microscopy
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    Chapter 32 Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species
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    Chapter 33 Flow Cytometric Analysis of Cell Surface Antigen Density
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    Chapter 34 Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry
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    Chapter 35 Flow Cytometric Methods of Analyzing Apoptotic Cells
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    Chapter 36 Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67
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    Chapter 37 Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads
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    Chapter 38 Preparation and Use of Nonradioactive Hybridization Probes
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    Chapter 39 Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization
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    Chapter 40 Biotinylated Probes in Colony Hybridization
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    Chapter 41 Nonisotopic In Situ Hybridization
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    Chapter 42 Expression of Recombinant Antibody Fusion Proteins in E. coli
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    Chapter 43 Expression of Antibody Fusion Proteins in Mammalian Cells
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    Chapter 44 Antibody Screening of Bacteriophage λ gt-11 DNA Expression Libraries
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    Chapter 45 Display of Antibody Chains on Filamentous Bacteriophage
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    Chapter 46 Production of Human Fab Antibody Fragments from Phage Display Libraries
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    Chapter 47 Antibodies from Phage Display Libraries as Immunochemical Reagents
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Title
Immunochemical Protocols
Published by
Humana Press, February 2008
DOI 10.1007/978-1-59259-257-9
ISBNs
978-0-89603-493-8, 978-1-59259-257-9
Editors

Pound, John D.

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