Chapter title |
Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-Photon Photoconversion
|
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Chapter number | 5 |
Book title |
Germinal Centers
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7095-7_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7094-0, 978-1-4939-7095-7
|
Authors |
Imogen Moran, Tri Giang Phan, Moran, Imogen, Phan, Tri Giang |
Abstract |
The germinal center (GC) reaction is the key process for the generation of high affinity antibodies to foreign antigen. Standard experimental techniques such as fluorescence-activated cell sorting and histology have provided numerous insights into the composition and function of the GC. However, these approaches are limited to a "snapshot" in time and are unable to fully capture the dynamic nature of the GC. Intravital two-photon microscopy overcomes these disadvantages and has led to several major advances in the field but is restricted by practical and technical limits that prevent long-range mapping and molecular studies. Here we describe procedures for optical marking or "tagging" of cells in precise microanatomical compartments by two-photon photoconversion that can be used for long-term fate mapping and transcript profiling of GC T and B cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 2 | 40% |
Researcher | 2 | 40% |
Student > Ph. D. Student | 1 | 20% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 2 | 40% |
Immunology and Microbiology | 1 | 20% |
Medicine and Dentistry | 1 | 20% |
Engineering | 1 | 20% |