Chapter title |
Antibody array generation and use.
|
---|---|
Chapter number | 36 |
Book title |
Monoclonal Antibodies
|
Published in |
Methods in molecular biology, February 2014
|
DOI | 10.1007/978-1-62703-992-5_36 |
Pubmed ID | |
Book ISBNs |
978-1-62703-991-8, 978-1-62703-992-5
|
Authors |
Borrebaeck CA, Wingren C, Carl A. K. Borrebaeck, Christer Wingren, Borrebaeck, Carl A. K., Wingren, Christer |
Abstract |
Affinity proteomics, represented by antibody arrays, is a multiplex technology for high-throughput protein expression profiling of crude proteomes in a highly specific, sensitive, and miniaturized manner. The antibodies are individually deposited in an ordered pattern, an array, onto a solid support. Next, the sample is added, and any specifically bound proteins are detected and quantified using mainly fluorescence as the mode of detection. The binding pattern is then converted into a relative protein expression map, or protein atlas, delineating the composition of the sample at the molecular level. The technology provides unique opportunities for various applications, such as protein expression profiling, biomarker discovery, disease diagnostics, prognostics, evidence-based therapy selection, and disease monitoring. Here, we describe the generation and use of planar antibody arrays for serum protein profiling. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 25% |
Student > Master | 1 | 25% |
Unknown | 2 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 25% |
Medicine and Dentistry | 1 | 25% |
Unknown | 2 | 50% |