| Chapter title |
Quantitative Investigation of Protein–Nucleic Acid Interactions by Biosensor Surface Plasmon Resonance
|
|---|---|
| Chapter number | 20 |
| Book title |
DNA-Protein Interactions
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2877-4_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2876-7, 978-1-4939-2877-4
|
| Authors |
Shuo Wang, Gregory M. K. Poon, W. David Wilson, Wang, Shuo, Poon, Gregory M. K., Wilson, W. David |
| Abstract |
Biosensor-surface plasmon resonance (SPR) technology has emerged as a powerful label-free approach for the study of nucleic acid interactions in real time. The method provides simultaneous equilibrium and kinetic characterization for biomolecular interactions with low sample requirements and without the need for external probes. A detailed and practical guide for protein-DNA interaction analyses using biosensor-SPR methods is presented. Details of SPR technology and basic fundamentals are described with recommendations on the preparation of the SPR instrument, sensor chips and samples, experimental design, quantitative and qualitative data analyses and presentation. A specific example of the interaction of a transcription factor with DNA is provided with results evaluated by both kinetic and steady-state SPR methods. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Unknown | 36 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 7 | 19% |
| Researcher | 7 | 19% |
| Student > Ph. D. Student | 6 | 16% |
| Student > Master | 3 | 8% |
| Student > Doctoral Student | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 10 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 15 | 41% |
| Agricultural and Biological Sciences | 4 | 11% |
| Chemistry | 2 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 12 | 32% |