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Nuclear Reprogramming

Overview of attention for book
Cover of 'Nuclear Reprogramming'

Table of Contents

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    Book Overview
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    Chapter 1 Nuclear Transfer in the Mouse
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    Chapter 2 Nuclear Transfer in Rabbit
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    Chapter 3 Nuclear Transfer in Ruminants
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    Chapter 4 Nuclear Transfer and Transgenesis in the Pig
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    Chapter 5 Nuclear Reprogramming
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    Chapter 6 Analysis of nuclear reprogramming following nuclear transfer to Xenopus oocyte.
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    Chapter 7 Assessing the Quality of Donor Cells: Karyotyping Methods
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    Chapter 8 Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer
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    Chapter 9 Fluorescent immunodetection of epigenetic modifications on preimplantation mouse embryos.
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    Chapter 10 Visualization of epigenetic modifications in preimplantation embryos.
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    Chapter 11 Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer
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    Chapter 12 Analysis of nucleolar morphology and protein localization as an indicator of nuclear reprogramming.
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    Chapter 13 Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining
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    Chapter 14 Gene Expression Analysis in Early Embryos Through Reverse Transcription Quantitative PCR (RT-qPCR)
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    Chapter 15 Studying Bovine Early Embryo Transcriptome by Microarray
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    Chapter 16 Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level
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    Chapter 17 Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos.
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    Chapter 18 Assessing reprogramming by chimera formation and tetraploid complementation.
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    Chapter 19 Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology
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    Chapter 20 Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
Attention for Chapter 18: Assessing reprogramming by chimera formation and tetraploid complementation.
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Chapter title
Assessing reprogramming by chimera formation and tetraploid complementation.
Chapter number 18
Book title
Nuclear Reprogramming
Published in
Methods in molecular biology, October 2014
DOI 10.1007/978-1-4939-1594-1_18
Pubmed ID
Book ISBNs
978-1-4939-1593-4, 978-1-4939-1594-1
Authors

Li X, Xia BL, Li W, Zhou Q, Xin Li, Bao-long Xia, Wei Li, Qi Zhou, Li, Xin, Xia, Bao-long, Li, Wei, Zhou, Qi

Abstract

Pluripotent stem cells can be evaluated by pluripotent markers expression, embryoid body aggregation, teratoma formation, chimera contribution and even more, tetraploid complementation. Whether iPS cells in general are functionally equivalent to normal ESCs is difficult to establish. Here, we present the detailed procedure for chimera formation and tetraploid complementation, the most stringent criterion, to assessing pluripotency.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Professor > Associate Professor 1 20%
Other 1 20%
Unknown 1 20%
Readers by discipline Count As %
Arts and Humanities 1 20%
Biochemistry, Genetics and Molecular Biology 1 20%
Agricultural and Biological Sciences 1 20%
Unknown 2 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2014.
All research outputs
#18,380,628
of 22,766,595 outputs
Outputs from Methods in molecular biology
#7,871
of 13,090 outputs
Outputs of similar age
#182,216
of 255,128 outputs
Outputs of similar age from Methods in molecular biology
#67
of 130 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,128 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 130 others from the same source and published within six weeks on either side of this one. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.