Chapter title |
Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling
|
---|---|
Chapter number | 8 |
Book title |
Selenoproteins
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7258-6_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7257-9, 978-1-4939-7258-6
|
Authors |
Brian K. Dalley, Lisa Baird, Michael T. Howard, Dalley, Brian K., Baird, Lisa, Howard, Michael T. |
Abstract |
Deep sequencing of ribosome protected mRNA footprints, also called ribosome profiling or Ribo-Seq, is a relatively new methodology well suited to address questions regarding the mechanisms and efficiency of protein expression. Specifically, the ability of this technique to quantify ribosome abundance with codon resolution enables experiments aimed at studying many aspects of translation, including gene-specific translational efficiency, translation of regulatory upstream short open reading frames, sites of ribosome pausing, and most importantly for selenoproteins, the efficiency by which UGA codons are redefined to encode selenocysteine. Here, we describe a streamlined protocol that was developed in our lab to process mammalian tissue to produce the requisite matched ribosome profiling and RNA-Seq libraries for deep sequencing. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Ireland | 1 | 25% |
France | 1 | 25% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 30% |
Other | 1 | 10% |
Student > Bachelor | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Professor > Associate Professor | 1 | 10% |
Other | 1 | 10% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 50% |
Agricultural and Biological Sciences | 2 | 20% |
Chemistry | 1 | 10% |
Unknown | 2 | 20% |