Chapter title |
Detection of mouse hepatitis virus nonstructural proteins using antisera directed against bacterial viral fusion proteins.
|
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Chapter number | 40 |
Book title |
Coronaviruses and their Diseases
|
Published in |
Advances in experimental medicine and biology, January 1990
|
DOI | 10.1007/978-1-4684-5823-7_40 |
Pubmed ID | |
Book ISBNs |
978-1-4684-5825-1, 978-1-4684-5823-7
|
Authors |
Zoltick, P W, Leibowitz, J L, DeVries, J, Pachuk, C J, Weiss, S R, Philip W. Zoltick, Julian L. Leibowitz, James DeVries, Catherine J. Pachuk, Susan R. Weiss, Zoltick, Philip W., Leibowitz, Julian L., DeVries, James, Pachuk, Catherine J., Weiss, Susan R. |
Abstract |
Mouse hepatitis virus, strain A59 cDNAs were inserted into the procaryotic fusion vector pGE374. RecA/viral/LacZ tripartite fusion proteins were synthesized from these plasmids and purified from E. coli. Antisera were raised in rabbits against these fusion proteins. Viral nonstructural proteins were detected in infected murine fibroblasts and glial cells. The anti-gene B, ORF1 sera detect a 30K cytoplasmic protein while the anti-gene E, ORF2 sera detect a 9.6K protein. Sera raised against proteins encoded in cDNAs from 5' portions of gene A immunoprecipitate the 200-250K polypeptides synthesized in vitro from genome RNA. Antisera raised against proteins encoded in both 5' and 3' portions of gene A immunoprecipitate membrane associated polypeptides of 150K and greater than 600K from MHV infected cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 10% |
Unknown | 9 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 2 | 20% |
Student > Bachelor | 2 | 20% |
Researcher | 2 | 20% |
Lecturer | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 30% |
Agricultural and Biological Sciences | 2 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 10% |
Unknown | 4 | 40% |