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Mendeley readers
Chapter title |
HIV-1 Reverse Transcriptase-Based Assay to Determine Cellular dNTP Concentrations
|
---|---|
Chapter number | 5 |
Book title |
HIV Protocols
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3046-3_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3045-6, 978-1-4939-3046-3
|
Authors |
Joseph A. Hollenbaugh, Baek Kim, Hollenbaugh, Joseph A., Kim, Baek |
Abstract |
Deoxynucleoside triphosphates (dNTPs) are the building blocks of DNA and their biosynthesis is tightly regulated in the cell. HPLC-MS and enzyme-based methods are currently employed to determine dNTP concentrations from cellular extracts. Here, we describe a highly efficient, HIV-1 reverse transcriptase (RT)-based assay to quantitate dNTP concentrations. The assay is based on the ability of HIV-1 RT to function at very low dNTP concentrations, thus providing for the high sensitivity of detection. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 17% |
Professor > Associate Professor | 2 | 17% |
Other | 1 | 8% |
Student > Master | 1 | 8% |
Professor | 1 | 8% |
Other | 2 | 17% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 3 | 25% |
Biochemistry, Genetics and Molecular Biology | 2 | 17% |
Immunology and Microbiology | 2 | 17% |
Engineering | 1 | 8% |
Unknown | 4 | 33% |