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The Nucleus

Overview of attention for book
Cover of 'The Nucleus'

Table of Contents

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    Book Overview
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    Chapter 1 Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals.
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    Chapter 2 Lysis Gradient Centrifugation: A Flexible Method for the Isolation of Nuclei from Primary Cells
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    Chapter 3 Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm
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    Chapter 4 A New Rapid Method for Isolating Nucleoli
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    Chapter 5 Sequential recovery of macromolecular components of the nucleolus.
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    Chapter 6 Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus
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    Chapter 7 Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling
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    Chapter 8 Purification of specific chromatin Loci for proteomic analysis.
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    Chapter 9 Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy.
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    Chapter 10 Purification of Proteins on Newly Synthesized DNA Using iPOND.
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    Chapter 11 Applying the ribopuromycylation method to detect nuclear translation.
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    Chapter 12 Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy
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    Chapter 13 A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox Dynamics
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    Chapter 14 Determination of the Dissociation Constant of the NFκB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy
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    Chapter 15 Imaging and quantification of amyloid fibrillation in the cell nucleus.
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    Chapter 16 Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images
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    Chapter 17 Quantitative Analysis of Chromosome Localization in the Nucleus
Attention for Chapter 11: Applying the ribopuromycylation method to detect nuclear translation.
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Chapter title
Applying the ribopuromycylation method to detect nuclear translation.
Chapter number 11
Book title
The Nucleus
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1680-1_11
Pubmed ID
25311127
Book ISBNs
978-1-4939-1679-5, 978-1-4939-1680-1
Authors

Alexandre David, Jonathan W Yewdell, Jonathan W. Yewdell, David, Alexandre, Yewdell, Jonathan W.

Abstract

Protein translation in the nucleus has been controversial for more than four decades. To take a new look at this potentially important phenomenon, we adapted the RiboPuromycylation Method (RPM) which labels actively translating ribosomes in cells via standard immunofluorescence microscopy. RPM is based on puromycylation of nascent chains trapped on ribosomes by antibiotics which inhibit chain elongation, followed by cell permeabilization/fixation and detection of puromycylated nascent chains using a puromycin-specific monoclonal antibody. To adapt the method to the nucleus, we use NP-40 rather than digitonin to permeabilize cells because NP-40 enables better antibody penetration into the nucleoplasm and particularly the nucleoli, a region of high translation as shown by RPM.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 31 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 23%
Researcher 4 13%
Student > Master 4 13%
Professor 3 10%
Professor > Associate Professor 3 10%
Other 7 23%
Unknown 3 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 32%
Agricultural and Biological Sciences 8 26%
Immunology and Microbiology 4 13%
Neuroscience 3 10%
Unspecified 1 3%
Other 2 6%
Unknown 3 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 October 2014.
All research outputs
#15,307,723
of 22,766,595 outputs
Outputs from Methods in molecular biology
#5,319
of 13,090 outputs
Outputs of similar age
#208,763
of 352,895 outputs
Outputs of similar age from Methods in molecular biology
#343
of 996 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,895 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.

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