Chapter title |
Sequence elements involved in the rescue of IBV defective RNA CD-91.
|
---|---|
Chapter number | 32 |
Book title |
Coronaviruses and Arteriviruses
|
Published in |
Advances in experimental medicine and biology, January 1998
|
DOI | 10.1007/978-1-4615-5331-1_32 |
Pubmed ID | |
Book ISBNs |
978-1-4613-7432-9, 978-1-4615-5331-1
|
Authors |
Dalton, K, Penzes, Z, Wroe, C, Stirrups, K, Evans, S, Shaw, K, Brown, T D, Britton, P, Cavanagh, D, Kevin Dalton, Z. Penzes, C. Wroe, K. Stirrups, S. Evans, K. Shaw, T. D. K. Brown, P. Britton, D. Cavanagh, Dalton, Kevin, Penzes, Z., Wroe, C., Stirrups, K., Evans, S., Shaw, K., Brown, T. D. K., Britton, P., Cavanagh, D. |
Abstract |
Deletion mutagenesis has been used to identify essential regions for rescue of coronavirus defective RNAs (D-RNAs). Using this technique on a cloned IBV D-RNA CD-91, we have identified a region potentially important in its rescue. Comparing the sequence of D-RNAs rescued with those not rescued we have deduced that a 72 base region corresponding to base number 13,824 to 13,896 in the viral genome is required for rescue. This may be an IBV D-RNA packaging signal or a cis-acting element involved in replication. Further experiments and modification of our techniques will be required to differentiate between the two processes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 55% |
Lecturer | 1 | 9% |
Librarian | 1 | 9% |
Student > Master | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Other | 0 | 0% |
Unknown | 1 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 36% |
Veterinary Science and Veterinary Medicine | 3 | 27% |
Nursing and Health Professions | 1 | 9% |
Agricultural and Biological Sciences | 1 | 9% |
Medicine and Dentistry | 1 | 9% |
Other | 0 | 0% |
Unknown | 1 | 9% |