Techniques in Molecular Biology

Table of Contents

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   Book Overview
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   Chapter 1 High Performance Liquid Chromatography of Proteins and Nucleic Acids
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   Chapter 2 Analytical Electrophoretic Techniques in Protein Chemistry
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   Chapter 3 Protein Blotting
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   Chapter 4 The Production and Isolation of Peptides for Sequence Analysis
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   Chapter 5 Protein and Peptide Sequence Determination
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   Chapter 6 The Extraction and Fractionation of RNA
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   Chapter 7 Protein Synthesis in vitro
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   Chapter 8 Labelling DNA in vitro — Nick Translation
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    Chapter 9 Synthesis of cDNA for Molecular Cloning
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    Chapter 10 Plasmid Isolation
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    Chapter 11 The Use of Restriction Endonucleases and T4 DNA Ligase
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    Chapter 12 Molecular Cloning of cDNA: Bacterial Transformation and Screening of Transformants
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    Chapter 13 The Use of Minicells and Maxicells to Detect the Expression of Cloned Genes
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    Chapter 14 Gel Electrophoresis of DNA
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    Chapter 15 Detection of Specific DNA Sequences — The Southern Blot
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    Chapter 16 The Determination of DNA Sequences
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    Chapter 17 The Use of Cosmids as Cloning Vehicles