Chapter title |
Synergistic interaction of tubby and tubby-like protein 1 (tulp1).
|
---|---|
Chapter number | 64 |
Book title |
Retinal Degenerative Diseases
|
Published in |
Advances in experimental medicine and biology, March 2014
|
DOI | 10.1007/978-1-4614-3209-8_64 |
Pubmed ID | |
Book ISBNs |
978-1-4614-3208-1, 978-1-4614-3209-8
|
Authors |
Caberoy NB, Nora Blanca Caberoy, Caberoy, Nora Blanca |
Abstract |
Mutations in either tubby or tubby-like protein 1 (Tulp1) cause retinal degeneration with undefined mechanisms. We recently identified both proteins with unconventional secretion as novel MerTK-specific phagocytosis ligands for retinal pigment epithelium (RPE) cells. Using our newly-developed open reading frame (ORF) phage display as a technology for protein-protein interactions, we identified Tulp1 as a Tubby-binding protein. The interaction of tubby and Tulp1 was verified by yeast two-hybrid and protein pull-down assays. Tubby and Tulp1 form heterodimer or heterooligomer and their interaction was functionally revealed by their synergistic stimulation of RPE phagocytosis. Tubby and Tulp1 mediated phagocytosis through MerTK-dependent signaling with non-muscle myosin II redistribution leading to colocalization of phagocytosed vesicles with rearranged NMMIIA. |
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